Inhibitory effect of NH4Cl on secretion of collagen in human gingival fibroblasts
Identifieur interne : 003600 ( Main/Exploration ); précédent : 003599; suivant : 003601Inhibitory effect of NH4Cl on secretion of collagen in human gingival fibroblasts
Auteurs : Kristen Helgeland [Norvège]Source :
- European Journal of Oral Sciences [ 0909-8836 ] ; 1984-10.
English descriptors
- Teeft :
- Amino, Amino acid pool, Amino acids, Ammonia, Ascorbic acid, Biochim biophys, Biol chem, Cell cultures, Cell layer, Cell type, Cellular fraction, Cellular pool, Chloroquine, Collagen, Collagen metabolism, Collagen secretion, Collagen synthesis, Control cultures, Control values, Cultured fibroblasts, Different lysosomotropic agents, Embryonic chick tendon cells, Extracellular collagen, Fibroblast, Fibronectin, General protein synthesis, Gingival, Human gingival fibroblasts, Inhibitory effect, Intracellular degradation, Methylamine, Nh4ci, Nh4ci treatment, Noncollagen, Noncollagen protein, Other proteins, Parallel cultures, Precursor pool, Prominent effect, Protein synthesis, Radiolabeled protein, Secretion.
Abstract
Abstract – The general protein synthesis in human gingival fibroblasts as measured by 14C‐proline incorporation was only moderately inhibited by 10 mM NH4Cl during incubation for 36 h. The proportion secreted as noncollagen protein and recovered from the cellular fraction as collagen was not significantly affected, whereas a pronounced inhibitory effect on the secretion of collagen was evident after 24 h. This effect was dose dependent, with a significant inhibition of collagen secretion even at 2 mM ammonia. The applied concentrations of NH4Cl had no significant effect on the hydroxylation of prolyl residues in collagen. Ammonia had no inhibitory effect on the secretion of fibronectin, another major secretory protein from fibroblasts. When comparing different lysosomotropic agents; NH4Cl, chloroquine and methylamine, the most prominent effect was consistently found to be an inhibition of the secretion of collagen.
Url:
DOI: 10.1111/j.1600-0722.1984.tb00911.x
Affiliations:
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Inhibitory effect of NH4Cl on secretion of collagen in human gingival fibroblasts</title><author><name sortKey="Helgeland, Kristen" sort="Helgeland, Kristen" uniqKey="Helgeland K" first="Kristen" last="Helgeland">Kristen Helgeland</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:160100910C7F32CDDAB0369946BEABAF77C73932</idno><date when="1984" year="1984">1984</date><idno type="doi">10.1111/j.1600-0722.1984.tb00911.x</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-BK37GLL1-3/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000695</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000695</idno><idno type="wicri:Area/Istex/Curation">000695</idno><idno type="wicri:Area/Istex/Checkpoint">002360</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002360</idno><idno type="wicri:doubleKey">0909-8836:1984:Helgeland K:inhibitory:effect:of</idno><idno type="wicri:Area/Main/Merge">003674</idno><idno type="wicri:Area/Main/Curation">003600</idno><idno type="wicri:Area/Main/Exploration">003600</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Inhibitory effect of NH4Cl on secretion of collagen in human gingival fibroblasts</title><author><name sortKey="Helgeland, Kristen" sort="Helgeland, Kristen" uniqKey="Helgeland K" first="Kristen" last="Helgeland">Kristen Helgeland</name><affiliation wicri:level="3"><country xml:lang="fr">Norvège</country><wicri:regionArea>Department of Microbiology, Dental Faculty, University of Oslo, Blindern, Oslo</wicri:regionArea><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Correspondence address: Department of Microbiology, Dental Faculty, University of Oslo, P.O.B. 1052, Blindern, Oslo 3</wicri:regionArea><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">European Journal of Oral Sciences</title><title level="j" type="alt">EUROPEAN JOURNAL OF ORAL SCIENCES</title><idno type="ISSN">0909-8836</idno><idno type="eISSN">1600-0722</idno><imprint><biblScope unit="vol">92</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="419">419</biblScope><biblScope unit="page" to="425">425</biblScope><biblScope unit="page-count">7</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1984-10">1984-10</date></imprint><idno type="ISSN">0909-8836</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0909-8836</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acid pool</term><term>Amino acids</term><term>Ammonia</term><term>Ascorbic acid</term><term>Biochim biophys</term><term>Biol chem</term><term>Cell cultures</term><term>Cell layer</term><term>Cell type</term><term>Cellular fraction</term><term>Cellular pool</term><term>Chloroquine</term><term>Collagen</term><term>Collagen metabolism</term><term>Collagen secretion</term><term>Collagen synthesis</term><term>Control cultures</term><term>Control values</term><term>Cultured fibroblasts</term><term>Different lysosomotropic agents</term><term>Embryonic chick tendon cells</term><term>Extracellular collagen</term><term>Fibroblast</term><term>Fibronectin</term><term>General protein synthesis</term><term>Gingival</term><term>Human gingival fibroblasts</term><term>Inhibitory effect</term><term>Intracellular degradation</term><term>Methylamine</term><term>Nh4ci</term><term>Nh4ci treatment</term><term>Noncollagen</term><term>Noncollagen protein</term><term>Other proteins</term><term>Parallel cultures</term><term>Precursor pool</term><term>Prominent effect</term><term>Protein synthesis</term><term>Radiolabeled protein</term><term>Secretion</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract – The general protein synthesis in human gingival fibroblasts as measured by 14C‐proline incorporation was only moderately inhibited by 10 mM NH4Cl during incubation for 36 h. The proportion secreted as noncollagen protein and recovered from the cellular fraction as collagen was not significantly affected, whereas a pronounced inhibitory effect on the secretion of collagen was evident after 24 h. This effect was dose dependent, with a significant inhibition of collagen secretion even at 2 mM ammonia. The applied concentrations of NH4Cl had no significant effect on the hydroxylation of prolyl residues in collagen. Ammonia had no inhibitory effect on the secretion of fibronectin, another major secretory protein from fibroblasts. When comparing different lysosomotropic agents; NH4Cl, chloroquine and methylamine, the most prominent effect was consistently found to be an inhibition of the secretion of collagen.</div></front></TEI><affiliations><list><country><li>Norvège</li></country><region><li>Østlandet</li></region><settlement><li>Oslo</li></settlement></list><tree><country name="Norvège"><region name="Østlandet"><name sortKey="Helgeland, Kristen" sort="Helgeland, Kristen" uniqKey="Helgeland K" first="Kristen" last="Helgeland">Kristen Helgeland</name></region><name sortKey="Helgeland, Kristen" sort="Helgeland, Kristen" uniqKey="Helgeland K" first="Kristen" last="Helgeland">Kristen Helgeland</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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